60108
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- 中文名稱: 肌酸酶AE
- 英文名稱: Creatinase (C2-AE)
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
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- 應 用: 生化診斷
- 產品類別: 酶
- 生化診斷: 比色法
規格
| Appearance | white to light yellow lyophilizate | |
| Activity | ≧9 U/mg | |
| Stabilizer | sucrose | |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 80 kDa (gel filtration) |
| Structure | 2 subunits of 46 kDa (SDS-PAGE) |
| Michaelis constant | 1.3×10-2M (creatine) |
| pH Optimum | 7.0–9.0 |
| pH Stability | 5.0–11.0 |
| Optimum temperature | 40℃ |
| Thermal stability | below 45℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Hg2+ |
應用
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
參考文獻
Suzuki, M, Medical Technology, 7, 945–950 (1979).
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.
